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Image Search Results
Journal: Cell death and differentiation
Article Title: Aberrant paracrine signalling for bone remodelling underlies the mutant histone-driven giant cell tumour of bone.
doi: 10.1038/s41418-022-01031-x
Figure Lengend Snippet: Fig. 5 SCUBE3 regulates osteoclast formation. A Representative photomicrographs on day 9 of osteoclasts generated from monocytes; treatment with rSCUBE3 results in fewer osteoclasts identifed by TRAP and vitronectin receptor (VNR) staining. B Dose-dependent inhibition of the total number of TRAP-positive osteoclasts by rSCUBE3; number relative to day 9 control cultures; 5 osteoclast preparations. C Quantification of number of nuclei per osteoclast following treatment with 1.25 μg/ml rSCUBE3, expressed as a proportion of OCs exhibiting 3–5 or ≥6 nuclei over the total number of OCs; 5 osteoclast preparations. D Number of osteoclasts generated in the presence of conditioned medium (CM) from differentiated (day 6) hFOBs in the presence or absence of 1.25 μg/ml rSCUBE3 (relative number to control H3.3WT CM); 20 osteoclast preparations. Data represent the mean ± SEM. B, C: 2-way RM ANOVA. D: 1-way ANOVA.
Article Snippet: Effects of rSCUBE3 were investigated by treatment of osteoclast cultures with 0.25–1.25 μg/ml
Techniques: Generated, Staining, Inhibition, Control